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KMID : 0881720090240020148
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Journal of Food Hygiene and Safety
2009 Volume.24 No. 2 p.148 ~ p.153
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Development of Total Cholesterol Detection System by Fluorescence Chromatography
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Oh Sang-Wook
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Abstract
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In this study, we have developed a fluorescence chromatographic assay for the quantification of total cholesterol in serum, which is a well-known risk predictor for cardiovascular diseases. The new assay system consists of a chromatographic strip in a cartridge, enzyme buffer containing cholesterol esterase, cholesterol oxidase, horseradish peroxidase, and color developer AEC, and a laser fluorescence scanner. The correlation coefficient (r) between cholesterol concentration and relative fluorescence units was 0.968 in the new assay, showing a reliable linearity through the tested range of cholesterol. Recovery test and comparability with a Hitachi 747 instrument showed 106.5-94% and r = 0.939 (p<0.001), respectively. The new assay system for cholesterol was developed as a pre-POCT platform conducted in clinics since it is fast (8 min) and uses a small volume of sample (), and it may be applied for on-site diagnostics to replace expensive automated biochemical analyzer.
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KEYWORD
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Total cholesterol, Fluorescence, Chromatographic assay, Laser fluorescence scanner
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